sheather sucrose flotation concentration research 2007

Journal of Parasitology   BioOne

Journal of Parasitology BioOne

The supernatant was discarded and the tubes were filled with sucrose flotation solution. The tubes were topped with an extra drop of sucrose solution and overlaid with a cover slip. These tubes were centrifuged at 10,500 g for 5 min to facilitate the flotation of eggs to the surface and their adherence to

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Paleoparasitological analysis of human remains from a

Paleoparasitological analysis of human remains from a

Sucrose flotation was conducted at a sucrose density of 1.2 (Sheather, 1923, modified). At least 3 slides were examined after 15, 45, and 105 min flotation or until no further structures were recovered.

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Enteric parasitic infection among HIV infected patients

Enteric parasitic infection among HIV infected patients

The fecal samples were processed by direct smear technique, in both normal saline solution and 1 % iodine solution as well as modified acid fast staining (Kinyouns method) after formalin ether concentration and Sheathers sucrose flotation for the identification of enteric parasites.

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Cryptosporidia Adult and Adolescent Opportunistic Infection

Cryptosporidia Adult and Adolescent Opportunistic Infection

Jun 14, 20130183;32;Concentration methods (i.e., formalin ether or formalin ethyl acetate) and flotation methods (i.e., Sheathers sucrose or sodium chloride) may facilitate diagnosis, but they are very labor intensive and not routinely used in clinical laboratories.

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Jundishapur Journal of Microbiology   Molecular Study of

Jundishapur Journal of Microbiology Molecular Study of

All samples were concentrated by sucrose flotation procedure (Sheathers method, with a specific gravity of 1.21), and thin smears of the concentrated layer of samples were then prepared on glass slides, air dried, and fixed with methanol, and stained by modified Ziehl Neelsen and investigated by

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Evaluation of the accuracy of parasitological techniques

Evaluation of the accuracy of parasitological techniques

Sheather technique was higher than that of the HPLJ technique for the diagnosis of Ancylostomatidae, because these parasites have light eggs that are more easily detected by flotation with density differences of the Sheather technique (SHEATHER, 1923; DE CARLI, 2001). The use of flotation techniques enables

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pyritic beneficiation equipment   vrolijk ts.nl

pyritic beneficiation equipment vrolijk ts.nl

Document Title [Title] The Beneficiation of Antimony Ore by Flotation B Kocaba, O antimony ore is the most important point for determining the area of usage. Inquiry; sheather sucrose flotation concentration research 2007

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The University of Adelaide  Public Health   Academia.edu

The University of Adelaide Public Health Academia.edu

The fecal samples were processed by direct smear technique, in both normal saline solution and 1 % iodine solution as well as modified acid fast staining (Kinyoun's method) after formalin ether concentration and Sheather's sucrose flotation for the identification of enteric parasites.

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Mehdi Azami  Isfahan University of Medical Sciences

Mehdi Azami Isfahan University of Medical Sciences

Stool specimens from renal transplant recipients and control groups were obtained between June 2006 and January 2007. The samples screened for intestinal parasitic infections using direct smear, formalin ether sedimentation, Sheather's flotation and modified Ziehl Neelsen staining methods.

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Comparison of current methods used to detect

Comparison of current methods used to detect

Flotation methods use a liquid suspending medium that is denser than the oocysts being concentrated. This can be used for the purpose of both concentration and purification. The most commonly used solution or media are sheather sucrose flotation (SSF), zinc sulphate flotation (ZSF) and saturated sodium chloride salt flotation (SSSF).

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Evaluation of the accuracy of parasitological techniques

Evaluation of the accuracy of parasitological techniques

For the diagnosis of Coccidia, flotation techniques using hypertonic solutions are recommended. Our results reinforce those of studies that demonstrate the accuracy achieved by associating the Willis and Sheather techniques, or even the HPLJ and Willis or Sheather techniques in routine laboratory tests (GARCIA et al., 1995; GARCIA, 2007).

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Methods for the in vitro culture of Sporozoea sp. and uses

Methods for the in vitro culture of Sporozoea sp. and uses

Jul 31, 20070183;32;Other methods to purify oocysts from samples that are commonly used include the Sheather sucrose flotation and Zinc sulfate flotation, e.g., see L R Ash and T C Orihel, Parasites A Guide to Laboratory Procedures and Identification, ASCP Press169; 1991, incorporated by reference herein.

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Prevalence and Molecular Epidemiology of Cryptosporidium

Prevalence and Molecular Epidemiology of Cryptosporidium

Fecal samples preparation Sheathers sucrose flotation method was used for the concentration of Cryptosporidium oocysts, concentrated samples were examined for the presence of oocysts by scanning of fecal smears on slides using the 40X objective lens of a bright field microscope and the presence of oocysts in the smear was confirmed under

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Loop Mediated Isothermal Amplification An Advanced Method

Loop Mediated Isothermal Amplification An Advanced Method

This chapter provides a reliable and quick method for detection of Giardia duodenalis (which causes a dangerous diarrheal disease), prevention of further spreading, identification of the source of contamination, and eventually minimize health risk and economic damage normally caused by an outbreak.

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Comparison of different diagnostic techniques for the

Comparison of different diagnostic techniques for the

Sheather's sugar flotation (SSF) method. The sucrose floatation was carried out by following standard procedure with Sheather's sugar solution of 1.26 specific gravity; centrifugation was done at 500 215;g for 10 min. Cryptosporidium oocysts were observed under magnification (215;40) and oil immersion (215;100) for observation of internal morphology.

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Enteric parasitic infection among HIV    BMC Research Notes

Enteric parasitic infection among HIV BMC Research Notes

The fecal samples were processed by direct smear technique, in both normal saline solution and 1 % iodine solution as well as modified acid fast staining (Kinyouns method) after formalin ether concentration and Sheathers sucrose flotation for the identification of enteric parasites.

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Enteric parasitic infection among HIV infected patients

Enteric parasitic infection among HIV infected patients

Teaching Hospital, Public Health Research Laboratory, Kathmandu, Nepal from July 2011 to June 2012. The fecal samples were processed by direct smear technique, in both normal saline solution and 1 % iodine solution as well as modified acid fast staining (Kinyouns method) after formalin ether concentration and Sheathers sucrose flotation for

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Prevalence and genotyping of Cryptosporidium isolated from

Prevalence and genotyping of Cryptosporidium isolated from

After the fecal material collection, Cryptosporidium oocysts from each sample were concentrated by Sheathers sucrose flotation technique and discontinuous sucrose gradient concentration.

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INVESTIGATION ON CRYPTOSPORIDIUM INFECTIONS IN WILD

INVESTIGATION ON CRYPTOSPORIDIUM INFECTIONS IN WILD

The pathogen examination was evaluated by Sheather's sugar flotation technique, 23 and modified acid fast staining. Briefly, saturated sucrose solution for Sheather's sugar flotation technique contains 500 g sucrose, 320 ml distilled water, and 6.7 ml phenol. This solution was added into the tube containing the feces.

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The University of Adelaide  Public Health   Academia.edu

The University of Adelaide Public Health Academia.edu

The fecal samples were processed by direct smear technique, in both normal saline solution and 1 % iodine solution as well as modified acid fast staining (Kinyoun's method) after formalin ether concentration and Sheather's sucrose flotation for the identification of enteric parasites.

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Evaluation of the accuracy of parasitological techniques

Evaluation of the accuracy of parasitological techniques

Sheather technique was higher than that of the HPLJ technique for the diagnosis of Ancylostomatidae, because these parasites have light eggs that are more easily detected by flotation with density differences of the Sheather technique (SHEATHER, 1923; DE CARLI, 2001). The use of flotation techniques enables

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Endoparasites in the feces of arctic foxes in a

Endoparasites in the feces of arctic foxes in a

Fecal flotation. A modified double centrifugation Sheathers sucrose flotation technique was performed on a known quantity (13 g) of feces from each sample. All ascarid eggs, observed by light microscopy, were counted and the number per gram of feces recorded. Other parasite eggs were not quantified on fecal flotation.

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Development of a nested polymerase chain reaction assay

Development of a nested polymerase chain reaction assay

Serial fecal samples collected during the next 38 days were tested via sucrose flotation and PCR assay. ResultsOocyst passage was detected via fecal flotation in all 4 alpacas 31 to 35 days after inoculation. Three had positive results for PCR assays on samples obtained 7

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Pcr Assays for Detection of Baylisascaris procyonis Eggs

Pcr Assays for Detection of Baylisascaris procyonis Eggs

The raccoon roundworm, B. procyonis, is an emerging public health concern in North America, Europe, and parts of Asia.This parasite is responsible for causing visceral, ocular, and/or neural larva migrans (NLM) in more than 100 species of birds and mammals, including humans.

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US20040018215A1   Methods for the in vitro culture of

US20040018215A1 Methods for the in vitro culture of

The invention relates to a method of excysting and growing protozoal oocysts by in vitro tissue culture resulting in production of a continuous culture of merozoites. The invention also provides an economical and reliable supply of cultured Eimeria sp. for vaccine production, assays and research.

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Assessment of reagent effectiveness and preservation

Assessment of reagent effectiveness and preservation

According to our results, the sucrose solution was the most suitable flotation solution for fresh samples (small strongyle egg per gram was 706 compared to 360 in sodium chlorid and 507 in sodium

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Cryptosporidium species detected in calves and cattle in

Cryptosporidium species detected in calves and cattle in

Before DNA extraction, Cryptosporidium 100 % identical to sequences in the database (Table 1). oocysts were concentrated by Sheather's sucrose flotation Two isolates of C. andersoni were 100 % identical to C. method (Ma and Soave 1983).

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Paleoparasitological analysis of human remains from a

Paleoparasitological analysis of human remains from a

Sucrose flotation was conducted at a sucrose density of 1.2 (Sheather, 1923, modified). At least 3 slides were examined after 15, 45, and 105 min flotation or until no further structures were recovered.

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Original article MOLECULAR EPIDEMIOLOGY OF

Original article MOLECULAR EPIDEMIOLOGY OF

2007). Moreover, C. parvum is the main Sheather's sucrose flotation method and oocysts were kept at 4 176;C in 2.5% potas sium dichromate till DNA extraction. Oo cysts were washed with 0.025M phos data are mostly uneven with concentration on humans cases only. Moreover, there is

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US7816118B2   Methods for the in vitro culture of

US7816118B2 Methods for the in vitro culture of

The invention relates to a method of excysting and growing protozoal oocysts by in vitro tissue culture resulting in production of a continuous culture of merozoites. The invention also provides an economical and reliable supply of cultured Eimeria sp. for vaccine production, assays and research. Domesticated avians that have been vaccinated using the provied Eimeria sp. are also provided.

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